Fig. 5

RT-qPCR validation for upregulated protein coding genes in the long-term treatment group. To validate the upregulated candidate genes on total RNA sequencing, the expression of MX1, KRT19, HMGA2, ISG15, IL33, MMP1, TRPA1, IFI6, PLAU, CDKN1A, PLAT, AK5 and NT5E was determined by RT-qPCR. All selected genes were verified to be increased in the long-term treatment group (within 35 days from 27 days). All experiments were performed 3 times with technical replicates