Fig. 6

RT-qPCR validation for downregulated protein coding genes in the long-term treatment group. To validate the upregulated candidate genes on total RNA sequencing, the expression of NDUFA4L2, SLITRK6, TIMP3, FMO3, HBG1, MGP, HBG2, TBX4, GPX3, and FMO2 was determined by RT-qPCR. All selected genes were verified to be decreased in the long-term treatment group. All experiments were performed 3 times with technical replicates