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Fig. 5 | BMC Pharmacology and Toxicology

Fig. 5

From: Metformin alleviates adriamycin resistance of osteosarcoma by declining YY1 to inhibit MDR1 transcriptional activity

Fig. 5

Overexpression of YY1 or MDR1 reversed the effect of Met on ADM sensitivity of MG-63/ADM cells. (A) The cytotoxic effects of Met treatment on ADM-induced apoptosis of MG-63/ADM cells after overexpression of YY1 or MDR1 were determined by flow cytometry. (B) The apoptosis rate was calculated. (C) Western blotting was performed to detect the protein levels P-gp, Bcl-2 and Bax in MG-63/ADM cells with YY1 or MDR1 overexpression. (D) Quantitative analysis of Western blot and GAPDH as internal reference. All the results were shown as mean ± SD (n = 3), which were three separate experiments performed in triplicate. **P < 0.01, ***P < 0.001

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BMC Pharmacology and Toxicology

ISSN: 2050-6511

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