Fig. 5

The AMPK inhibitor CC suppresses autophagy and apoptosis in RTAEC. RTAEC were treated with 10 μM CC for 2 h and subsequently exposed to 500 nM rotenone for 24 h. A Western blotting was used to detect the expression of P-AMPK/AMPK and P-ULK1/ULK1 proteins in RTAEC after CC treatment. B Western blotting was used to detect the expression of LC3, P62, and Beclin1 proteins in RTAEC after CC treatment. C Western blotting was used to detect the expression of Bax, Bcl-2, Caspase3, and Cleaved-Caspase3 proteins in RTAEC after CC treatment. D The apoptosis of RTAEC treated with CC was detected by flow cytometry. The results were expressed as the mean ± SD of three independent experiments. *P stands for P < 0.05; **P stands for P < 0.01