Table 6 Stability evaluation of SAX and 5-OH SAX (n = 3)
Analytes | Spiked concentration (ng/mL) | Freshly preparations (%) | Room temperature for 4 h (%) | 4 °C for 12 h (%) | −80 °C for 7 days (%) | Freeze-thaw three cycles (%) | Autosampler at 4 °C for up to 16 h (%) |
|---|---|---|---|---|---|---|---|
SAX | 15 | 100.62 ± 3.08 | 99.02 ± 2.02 | 100.60 ± 2.29 | 98.56 ± 2.14 | 96.71 ± 1.93 | 99.02 ± 2.00 |
2000 | 98.99 ± 2.10 | 99.59 ± 3.47 | 99.09 ± 2.02 | 98.07 ± 2.07 | 95.84 ± 1.52 | 98.05 ± 2.04 | |
5-OH SAX | 3 | 100.67 ± 4.16 | 99.78 ± 2.22 | 100.67 ± 2.52 | 95.11 ± 4.55 | 94.89 ± 2.91 | 100.44 ± 3.10 |
400 | 101.04 ± 2.71 | 98.51 ± 2.19 | 99.32 ± 2.66 | 97.49 ± 2.52 | 95.79 ± 2.16 | 101.22 ± 2.47 |