Fig. 5
Effect of LPS on ROS production in hFOB1.19 cells. (A) Flow cytometry analysis of hFOB1.19 cells. (B) Quantitative ROS analysis via flow cytometry. ROS increased in hFOB1.19 cells after 1, 3, and 5 days of LPS induction, and this increase was reversed by luteolin. Combined with LPS and H2O2 induction, ROS were significantly increased in hFOB1.19 cells after 1, 3, and 5 days. This effect was also alleviated by luteolin. (C) 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging assay of hFOB1.19 cells after 3 days. ABTS decreased in hFOB1.19 cells after 1, 3, and 5 days of LPS induction, and this decrease was reversed by luteolin. Combined with LPS and H2O2 induction, ROS were significantly decreased in hFOB1.19 cells after 3 days. This effect was also alleviated by luteolin. The data are expressed as the means ± SDs (***p < 0.001, **p < 0.01, *p < 0.05)