Fig. 7
Effect of luteolin on the protein expression of hFOB1.19 cells and its downstream targets. (A, C, E, G) Western blotting analysis of IL-6, IL-8, TNF-α, p-p65 and p-lκB-α. (B, D, F, H) Relative protein expression levels of IL-6, IL-8, TNF-α, p-p65, p65, lκB-α, and p-lκB-α. The protein levels of IL-6, IL-8, TNF-α, p-p65, and p-lκB-α were increased in human osteoblasts after 1, 3, and 5 days of LPS induction, and this increase was moderated by luteolin. Combined with LPS and H2O2 induction, IL-6, IL-8, and TNF-α expression significantly increased in human osteoblasts after 1, 3, and 5 days. This effect was also alleviated by luteolin. The data are expressed as the means ± SDs (***p < 0.001, **p < 0.01, *p < 0.05)