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Table 2 Mobile phase gradient setup

From: Passively administered fluoxetine reaches the juvenile brain of FSL rats and reduces antioxidant defences, without altering serotonin turnover

Step

Time (min)

A (%)

HPLC water / 0.1% FA

B (%)

ACN / 0.1% FA

1

Start condition 0

95.0

5.0

2

3.0

95.0

5.0

3

4.3

0.0

100.0

4

12.0

0.0

100.0

5

14.0

95.0

5.0

6

15.0

95.0

5.0

7

Post-time 3

95.0

5.0

  1. The mobile phase was made up of HPLC water, 0.1% (v/v) formic acid (FA) and 0.1% acetonitrile (ACN), with a 3-minute post-time running period included
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BMC Pharmacology and Toxicology

ISSN: 2050-6511

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