Fig. 1

Econazole-induced changes in [Ca²⁺]_i in mouse DRG neurons. (A) The chemical structures of econazole, miconazole, and fluconazole are shown. (B) The actual recordings of [Ca²⁺]_i responses to econazole at 10 µM (left) and 100 µM (right) and to 80 mM KCl in wild type mouse DRG neurons. (C) The relationship between the magnitude of [Ca²⁺]_i increase induced by econazole (Eco) and by KCl at different concentrations of econazole. (D) The percentages of the neurons responding to econazole at various concentrations are shown. Symbols or columns with vertical lines represent the mean values ± SEM for each econazole concentration (n = 193–395 from 4–6 mice). (E) The actual recording of [Ca²⁺]_i responses to 10 µM econazole in the absence of external Ca²⁺ (0Ca) and KCl is shown. The 0Ca period is indicated with a grayish bar. (F) A summary of the amplitudes of [Ca²⁺]_i responses to econazole at 10 and 30 µM in the presence (control) and absence of extracellular Ca²⁺ (0Ca) (n = 133–190 from three mice) is shown. **P < 0.01 vs. control, as determined by Student’s t-test