Fig. 4
The [Ca2+]i responses to miconazole and effects of TRP channel antagonists and gene deletion in mouse DRG neurons. (A) The relationship between the magnitude of [Ca2+]i increase induced by miconazole (Mico) and by KCl at different concentrations of miconazole. Symbols with vertical lines represent mean ± SEM (n = 132–231 from 3–6 mice). (B) A summary of the amplitudes of [Ca2+]i responses to 10 and 30 µM of miconazole in the presence (control) and absence of extracellular Ca2+ (0Ca) is shown (10 µM: control, n = 103; 0Ca, n = 127; 30 µM: control, n = 106; 0Ca, n = 93 from three mice). Some vertical lines are obscured by symbols. (C) The Venn diagram shows the number of neurons that responded to 10 µM miconazole (Mico), 0.1 µM AITC, 1 µM capsaicin (Cap), and 80 mM KCl. The outer area of all the circles indicates the number of neurons that responded to KCl alone. Data are from three mice. (D) A summary of [Ca2+]i responses to miconazole at 10 µM (left) and 30 µM (right) in the absence (Cont) or presence of 10 µM A967079 (A96) or 1 µM BCTC is shown. Columns with vertical lines represent the mean values ± SEM (10 µM miconazole, n = 120–201; 30 µM miconazole, n = 142–199 from four mice). **P < 0.01 vs. control, one-way ANOVA with Tukey–Kramer test. (E) A summary of [Ca2+]i responses to miconazole at 10 µM (left) and 30 µM (right) in wild type (Wild), TRPA1 knockout (A1(−/−)), and TRPV1 knockout (V1(−/−)) mouse DRG neurons is shown. Columns with vertical lines show the mean values ± SEM (10 µM miconazole: wild, n = 215; A1(−/−), n = 201; V1(−/−), n = 198 from four mice. 30 µM miconazole: wild, n = 223; A1(−/−), n = 198; V1(−/−), n = 220 from four mice). *P < 0.05 vs. wild, one-way ANOVA with Tukey–Kramer test