Fig. 5
[Ca2+]i responses to miconazole in naïve and TRPA1-expessed HEK 293 cells. (A) The actual recordings of [Ca2+]i responses to 30 µM miconazole in the presence (left) or absence of extracellular Ca2+ (0Ca) (right) in naïve HEK 293 cells are shown. The 0Ca period is indicated with a grayish bar. (B) A summary of the amplitudes of [Ca2+]i responses to miconazole at 10 µM (left) and 30 µM (right) in naïve-HEK cells in the presence (control) or absence (0Ca) of extracellular Ca2+ is shown. Columns with vertical lines represent the mean ± SEM (miconazole 10 µM: control, n = 103; 0Ca, n = 127; miconazole 30 µM: control, n = 106; 0Ca, n = 93 from three experiments). **P < 0.01 vs. control, as determined by Student’s t-test (C) The actual recordings of [Ca2+]i responses to 10 µM miconazole and 100 µM AITC in TRPA1-expressed HEK293 cells (upper, A1-HEK) and to 1 µM capsaicin (Cap) in TRPV1-expressed HEK cells (lower, V1-HEK) are shown. (D) The relationship between [Ca2+]i responses and the concentrations of miconazole in A1-HEK and naïve HEK cells is shown. Symbols with vertical lines represent the mean ± SEM (A1-HEK, n = 54–134; naïve-HEK, n = 298–333 from 3–4 experiments). **P < 0.01 vs. naïve-HEK, as determined by Student’s t-test