Fig. 6
[Ca2+]i responses to fluconazole in mouse DRG neurons and HEK293 cells expressing TRPA1. (A) The actual recordings of [Ca2+]i responses to the sequential application of 100 µM fluconazole, 0.1 mM AITC, 1 µM capsaicin (Cap), and 80 mM KCl in mouse DRG neurons depict four patterns of responses. (B) The representative changes in [Ca2+]i induced by 100 µM fluconazole in HEK293 cells expressing TRPA1 (left: A1-HEK) and TRPV1 (right: V1-HEK). To determine the expression of each channel, a specific agonist was applied as follows: 0.1 mM ATIC for TRPA1 and 1 µM capsaicin (Cap) for TRPV1. Similar results are obtained in the two additional experiments