Fig. 1
From: Curcumin suppresses copper accumulation in non-small cell lung cancer by binding ATOX1
Effects of curcumin on ATOX1-associated copper chaperones in A549 and H1299 cells. (A) The binding activity between curcumin and ATOX1 protein was analyzed using molecular docking. (B) Cell viability was measured by CCK-8. (C) Levels of ATOX1, ATP7A, and COX17 proteins were measured by western blotting. The levels of proteins were normalized by β-actin. The full-length blots were shown in Supplementary Fig. 1 and Fig. 2. (D) The semi-quantitative analysis of the blots was analyzed by ImageJ software. (E) Effects of DC-AC50 treatment on cell viability. Three parallel experiments were performed in cells. NS no significance. *p < 0.05, **p < 0.01