Fig. 2
SH significantly attenuated LPS-induced ferroptosis by inhibiting the TRAF6-c-Myc signaling pathway in vivo. (A) The level of Fe2+ was evaluated by a ferrous iron colorimetric assay kit. (B-C) The levels of GSH and MDA was detected by Elisa kits. (D-E) Western blotting was used to detect the expression of GPX4, FTH1, NOX1, NCOA4, TRAF6, and c-Myc protein. (F) The mRNA expression of GPX4, FTH1, NOX1, NCOA4, TRAF6, and c-Myc was detected by qRT-PCR. (G) Representative immunohistochemical images of the expression of the TRAF6 in the lung tissue (scar bar = 50 μm). (H) The statistics of immunohistochemical staining. (I) Detection of c-Myc protein expression level by immunofluorescence staining (scar bar = 50 μm, n = 4 for every group). Data are shown as the mean ± SD, #p < 0.05; ##p < 0.01; ###p < 0.001; ####p < 0.0001; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001