Fig. 5
A representative image of liver tissue with HE staining A control group within normal structure of the liver lobules, central vein, and surrounding hepatocytes is clearly visible, B CCl4 group show moderate to severe vacuolar degeneration (arrowhead) and multifocal necrosis (yellow arrow) with multifocal inflammatory cell infiltration (black arrow) and hemorrhage in between hepatocyte (star), C and D CS and FO groups show focal hepatic necrosis mixed with mononuclear inflammatory cells (arrow) surrounded with hepatocytes reveal marked vacuolation (arrowhead), E and F represent GSH and VCO show mild improvement of liver tissue within focal hepatic necrosis mixed with mononuclear inflammatory cells and diffuse moderate vacuolar changes within the hepatocytes (arrowhead) and presence of mitotic figure (circle), G, H Nano-CS and Nano-VCO show more improvement degree of hepatic tissue with presence of mitotic figure and mild infammatory cells infilterated, I represents conventional combination (O) of treatments show normal hepatic parenchyma with midzonal area of fatthy changes in hepatic parenchyma. J that represent nano-combination (Nano-O) treatments show more healthy hepatic tissue within mild degree of periportal hepatic vacuolation consistent with mild fatty change (arrowhead). Scale bar = 50 μm. CCl4 carbon tetrachloride, CS chitosan, GSH glutathione, Fe3O4 ferric oxide, VCO virgin coconut oil