Fig. 7

HTR4 downregulation in 3T3-L1 cells prevents palmitate-induced inflammasome complex formation. (A) Representative western blots (left) and densitometric analysis (right) of the indicated protein (n = 3) and (B) levels of TNF-α, IL-1β, and IL-6 in 3T3-L1 cell culture medium measured using an ELISA kit after 200 μM palmitate treatment in HTR4-downregulated 3T3-L1 cells (n = 6). A student’s t-test was performed. Error bars on plots represent standard deviation (SD); *p < 0.05, **p < 0.01, ***p < 0.001. Abbreviations ASC apoptosis-associated speck-like protein containing a caspase-recruitment domain, c-caspase-1 cleaved caspase-1, eIF2α eukaryotic translation initiation factor 2α, ELISA enzyme-linked immunosorbent assay, HTR serotonin receptor, IL interleukin, NLRP3 NOD-like receptor family pyrin domain containing 3, PERK protein kinase R-like endoplasmic reticulum kinase, TNF tumor necrosis factor