Fig. 8

HTR4 downregulation improves palmitate-induced insulin resistance in Hep3B cells, but not in 3T3-L1 cells. Representative western blots (left) and densitometric analysis (right) of the indicated protein upon insulin (1 nM) treatment after 200 μM palmitate treatment in HTR4-downregulated Hep3B (A) and 3T3-L1 cells (B) (n = 3) are shown. A student’s t-test was performed. Error bars on plots represent standard deviation (SD); *p < 0.05, **p < 0.01. All experiments were performed in triplicate. Abbreviations Akt protein kinase B, HTR serotonin receptor