Fig. 4

PEG35-enriched RLS prevents liver energy metabolism. Phosphorylated adenosine monophosphate-activated protein kinase protein (AMPK) expression by immunofluorescence (original magnification ×40, ×100) (A) and AMPK signal intensity (B) was evaluated in livers harvested from untreated rats and immediately submitted to 120 min of ex vivo reperfusion (Sham), in livers subjected to cold ischemia for 24 h at 4 °C and submitted to 120 min of ex vivo reperfusion (Cold IR), in livers subjected to cold ischemia for 24 h at 4 °C and rinsed with Ringer’s lactate solution before undergoing a 120-min of ex vivo reperfusion (RLS) and in livers subjected to cold ischemia for 24 h at 4 °C and rinsed with Ringer’s lactate solution enriched with PEG35 at 5 g/L before undergoing a 120-min of ex vivo reperfusion (RLS + PEG35). Data were expressed as means ± SD (n = 8 for each group). ^aP < 0.05 vs. Sham; ^bP < 0.05 vs. Cold IR; ^cP < 0.05 vs. RLS