Fig. 4

Mortality and hatching rate of zebrafish embryos exposed to different concentrations of 3-benzylidene-indolin-2-one. a) Mortality rate (%) of zebrafish embryos at 24, 48, 72, and 96 h of post fertilization (hpf). b) Hatching rate (%) of zebrafish embryos at 48 and 72 hpf. Data are presented as mean ± SEM (n = 20 embryos/group) of three independent replicates. (*Indicates the significant level when compare to the respective negative control group, * p < 0.05, **p < 0.01, *** p < 0.001) by statistical analysis using ANOVA followed up by Dunnet’s post hoc test)