Fig. 3

MEL/EVE therapies cause apoptosis and the accumulation of sub G1 cells in HR+, HER2- breast cancer cells. A The amount of apoptosis was measured using annexin V-FITC labeling to detect apoptotic cells in comparison to untreated cells (control), followed by flow cytometry analysis. Bar graphs depict the apoptosis rate of each group.B Flow cytometry was used to examine the cell cycle distribution of 72-hour melatonin, everolimus, and MEL/EVE-treated MCF-7 cells. Bar graphs depict the cell cycle distribution rate of each group. (Three independent experiments were performed). The bars represent mean ± SEM. *p < 0.05, **p < 0.005, ***p < 0.0005