Fig. 5

Respiration rates in digitonin-permeabilized MCF-7 cells. MCF-7 cells supplied with succinate (10 mM) in the presence of 0.2 mM ADP (state 3) A, 5 µM oligomycin A (state 4o) B, 100 mM FCCP (State 3u) C and RCR calculation (state 3/state 4) D were examined by Hansatech Oxygraph. E OCR in digitonin‐permeabilized MCF-7 cells with TMPD/ascorbate (0.2 mM/10 mM) was calculated Hansatech Oxygraph. (At least six independent experiments were performed). F Fluorescence pictures of cells labeled with JC-1 following treatment with the drugs in use (green damaged mitochondria, red healthy mitochondria). G. The amount of fluorescence intensity was measured with ımage J software and shown as bar graphs (At least ten independent experiments were performed). The bars represent mean ± SEM. *p < 0.05, **p < 0.005. OCR, oxygen consumption rate, RCR, respiratory control ratio; TMPD, N, N, N’, N’‐tetramethyl‐p‐phenylenediamine