Fig. 2

Kaempferol restrained H/R-induced cell pyroptosis. For identifying the effects of Kaempferol on cell pyroptosis, H9c2 cells stimulated with H/R to induce injury, and were administered with 20 µM Kaempferol (A) LDH release was measured with LDH detection assay. The concentrations of (B) IL-1β and (C) IL-18 were examined by ELISA assays. (D) Flow cytometry was carried out to analyze cell pyroptosis rate. (E) Western blot analysis of the expression of pyroptosis markers NLRP3, caspase 1-p20 and GSDMD-N. CON, control; H/R, hypoxia/reoxygenation; Kae, Kaempferol; LDH, lactate dehydrogenase; IL-1β, interleukin-1β; IL-18, interleukin-18; PI, propidium iodide; GSDMD-N, GSDMD-N-terminal domain. ^**P < 0.01. ^*P < 0.05