Fig. 4

Knockdown of OGT reversed the inhibitory effects of Kaempferol on H/R-induced pyroptosis. (A, B) The RT-qPCR and western blot detection of OGT expression in H9c2 cells transfected with si-nc or si-OGT#1/2. (C) LDH activity and the expression of (D) IL-1β and (E) IL-18 were examined by using corresponding kits. (F) The percent of pyroptotic cells was estimated by flow cytometry following different treatments. (G) The role of OGT in Kaempferol-mediated pyroptosis was also validated by western blot. si-OGT, small interfering RNA targeting OGT; si-nc, siRNA negative control; LDH, lactate dehydrogenase; IL-1β, interleukin-1β; IL-18, interleukin-18; PI, propidium iodide; GSDMD-N, GSDMD-N-terminal domain. ^*P < 0.05, ^**P < 0.01