Fig. 4

FTO effects on TLR4 mRNA levels. A qPCR analysis of TLR4 levels in HEK293T cells transfected with LV-shNC or LV-shFTO. B MeRIP-qPCR analysis of m6A modification of TLR4 levels in HEK293T cells transfected with LV-shNC or LV-shFTO. C mRNA levels of TLR4 in HEK293T cells treated with Actinomycin D were monitored at different time points. ***p < 0.001