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Fig. 5 | BMC Pharmacology and Toxicology

Fig. 5

From: Saikosaponin D inhibits the inflammatory response of secretory otitis media through FTO-mediated N6-methyladenosine modification of TLR4 mRNA

Fig. 5

SSD decreases TLR4 mRNA stability by suppressing FTO mediated m6A modification. A qPCR analysis of FTO levels in HEK293T cells transfected with LV-shFTO before and after SSD treatment. B&C Western blot analysis of FTO levels in HEK293T cells transfected with LV-shFTO before and after SSD treatment. D qPCR analysis of TLR4 levels in HEK293T cells transfected with LV-shFTO before and after SSD treatment. E MeRIP-qPCR analysis of m6A modification of TLR4 levels in HEK293T cells transfected with LV-shFTO before and after SSD treatment. F mRNA levels of TLR4 in HEK293T cells treated with Actinomycin D were monitored at different time points. **p < 0.01, ***p < 0.001

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BMC Pharmacology and Toxicology

ISSN: 2050-6511

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