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Fig. 3 | BMC Pharmacology and Toxicology

Fig. 3

From: Quercetin-mediated restoration of high-fat diet-induced male reproductive dysfunction through modifying spermatogenesis and unraveling 3β-HSD, 17β-HSD, and StAR pathways

Fig. 3

PCNA expression variability in testes of different rat groups. Immunohistochemical analysis of PCNA expression in the testes of various rat groups demonstrates highlighting distinct staining patterns and expression areas. In Fig. 3A, testes from control rats display notable nuclear PCNA antibody expression within the spermatogenic cell layers (arrowheads). Similarly, Fig. 3B shows testes from QRT-administered rats with marked nuclear immunostaining of PCNA antibody within the spermatogenic cell layers (arrowheads). Conversely, Figs. 3C1 and 3C2 depict testes from HFD-fed rats with a significant reduction in PCNA-positive spermatogenic cells within the seminiferous tubules (arrowheads). In Fig. 3D, testes from QRT + HFD-fed rats exhibit a pronounced increase in PCNA-positive spermatogenic cells within the seminiferous tubules (arrowheads). In Fig. 3E, PCNA expression was quantified as a percentage of positive expression in a total of 1000 cells per 8 high-power fields (HPF). The interventions include quercetin (QRT), a high-fat diet (HFD), and a combination of both (QRT + HFD). Values are presented as mean ± standard deviation (SD) (n = 7), and significant differences are denoted by different letters (P < 0.05)

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BMC Pharmacology and Toxicology

ISSN: 2050-6511

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