Fig. 1

Example of flow cytometry analysis and gating strategy for identifying and quantifying blood dendritic cell subsets. The PBMC (peripheral blood mononuclear cell) population is identified by a combination of forward/side scatter characteristics (A), and DC was identified within the lineage (CD3, CD14, CD16, CD19, CD20, CD56, and CD34)-negative (Lin−) HLA-DR+ (B). One representative experiment demonstrates the gating strategy for identifying DC subsets (C and D). mDCs: myeloid DCs (CD11c+), pDCs: plasmacytoid DCs (CD123+)