Table 1 Gradient elution program of the mobile phase of the optimized method
Time (Min) | Flow (ml/min) | Buffer (0.1% OPA) (ml) | Acetonitrile (ml) |
|---|---|---|---|
1.0 | 1.0 | 75 | 25 |
2.0 | 1.0 | 75 | 25 |
10.0 | 1.0 | 30 | 70 |
12.0 | 1.0 | 30 | 70 |
13.0 | 1.0 | 70 | 30 |
18.0 | 1.0 | 75 | 25 |
Time (Min) | Flow (ml/min) | Buffer (0.1% OPA) (ml) | Acetonitrile (ml) |
|---|---|---|---|
1.0 | 1.0 | 75 | 25 |
2.0 | 1.0 | 75 | 25 |
10.0 | 1.0 | 30 | 70 |
12.0 | 1.0 | 30 | 70 |
13.0 | 1.0 | 70 | 30 |
18.0 | 1.0 | 75 | 25 |